sábado, 11 de abril de 2020
Molecular Identification of Filamentous Fungi Diversity in North Coast Beaches Sands of Puerto Rico
The Northern region has a great variety of beaches with diverse microbial characteristics. Beach sands receive direct contamination from the garbage generated by people, which serves as nutrient for fungi growth. The objectives of this investigation were to assess the filamentous fungi diversity of four popular beaches; identify the genus and species; and identify the taxonomic relationship between the most abundant fungi. The beaches studied are located in the towns of Vega Baja, Manatí, Barceloneta and Arecibo. One sample of dry sand per month from three equidistant points was acquired every month for a year in each beach. The samples were homogenized according to dry (December-April) and humid (May-November) seasons, for a total of four composite samples per season. The DNA of each sample was isolated and quantified; and, upon sequencing, evaluated by metagenomics analysis with MG-RAST. There were 104 fungi species identified by DNA sequencing analysis. The most abundant were: Aspergillus penicillioides, Aspergillus terreus, Microascus sp., Arthrographis kalrae, Paramicrosporidium sp., Dokmaia sp., Gliomastix polychroma and Aspergillus sp. The taxonomic analysis demonstrated that there is no relationship in the genus of the most abundant species. As significant finding, 66 species of new registries were identified, including Malassezia restricta, Arthrographics eremomycyces, and Cephaliophora tropica. Not only were many of the species pathogenic, several genera of filamentous fungi have been previously isolated from patients in nasal culture, and can cause eye, respiratory and skin disease. The majority of these fungi use direct contact and air transport as transmission vehicle to the host.
Citation
Echevarría, L., 2019. Molecular Identification of Filamentous Fungi Diversity in North Coast Beaches Sands of Puerto Rico. Int. J. Mol. Microbiol., 2(3): 51-61
https://www.journals.psmpublishers.org/index.php/ijmm/article/view/313
https://www.journals.psmpublishers.org/index.php/ijmm/article/view/313
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viernes, 22 de marzo de 2019
Preliminary Study to identify Filamentous Fungi in Sands of
Three Beaches of the Caribbean
The Caribbean has several white sand beaches visited by many tourists, especially during the summer. The beaches of Copacabana in Barbados Bridgetown, Flamenco Beach in Culebra Puerto Rico, and Maho Beach in St. Maarten were studied. The sand samples were taken in June 2016. The objective of the study was to know the diversity and quantity of filamentous fungi in the dry sand of the beaches and to determine if the fungi found are pathogenic to animals and humans. One gram of each beach sample was taken in triplicate and spread on a plate with rose bengal agar. The samples were incubated for 7 to 14 days at 25°C. The colonies were counted, then isolated in tubes with potato dextrose agar. Three genus of fungi were found; Aspergillus, Penicillium, and Rhizopus. The Aspergillus species resulted in higher identifications in the three beaches included in this preliminary study. The growth of filamentous fungi in the samples ranged from 6 CFU / g to 17 CFU / g. The species were: A. niger, A. versicolor, A. flavus, A. oryzae, A. fumigatus, R. oligosporus, R. stolonifer, A. tamarii and P. wasksmanii. Most of the identified filamentous fungi are pathogenic to humans and animals. Some of the species can cause diseases such as asthma, infections in the eyes, skin, and nails.
Citation:
Echevarría, L., 2019. Preliminary Study to identify Filamentous Fungi in Sands of Three Beaches of the Caribbean. PSM Microbiol., 4(1): 1-6.
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